FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065896
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29340.27526137634440884No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG15200.14260303068967328TruSeq Adapter, Index 11 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4100.037.8705941
CTAGATG2300.026.99957769
GTCTTCT6650.025.42065449
ATCTTTT40900.025.3052277
GTATGCC7050.024.46770342
CCGTCTT7450.024.0801647
TCTTTTT43750.023.9721978
GATCTTT45650.023.956916
CTTCTGC7150.023.64298651
TATGCCG7400.023.31044643
CTTTTTT45100.022.949729
TCTTCTG7500.022.53964650
TGCCGTC7700.022.40224645
ACGTATG3400.022.32318169
CTCGTAT7900.022.27180539
TTCTGCT7750.021.81256152
TAACGAC1906.7484507E-1021.78913355
TGCTTGA8050.021.42823455
GTCGTAA2101.0186341E-1021.35781134
ATGCCGT8350.020.6583644
Produced by FastQC (version 0.11.2)