FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754435
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87830.5006170077546332No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG24530.13981709211227547TruSeq Adapter, Index 11 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22410.12773342985063568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATG4650.028.93557769
TTTCGGA8900.025.5916481
ACGTATG4650.023.74201269
ATCTTTT83000.023.6921757
GATCTTT88050.023.5087786
TATGCCG9300.023.37037843
CCGTCTT9200.022.87442447
TCTTTTT86850.022.5227498
GTATGCC9700.022.05099342
CTCGTAT10450.021.78892539
CTTTTTT90850.021.5703059
GTCTTCT10050.021.28365349
TGCCGTC10250.020.86776745
GCCGTCT10100.020.8361146
ACTGTGC1356.828197E-620.443938
CGATCTT138500.020.0269325
CTTCTGC11400.019.67111851
ATCTGTG8200.019.3531727
TGCTTGA11600.019.3319655
ATGCCGT11100.019.26978544
Produced by FastQC (version 0.11.2)