FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687608
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48450.2870927371759318No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG36450.2159861768846794TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG17830.1056524974994193TruSeq Adapter, Index 11 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC12300.033.9379342
TTTCGGA4900.033.0986181
CCGTCTT13200.031.88533647
GTCTTCT13350.031.78643449
ATGCCGT13600.031.201244
TGCCGTC14100.031.07346745
GCCGTCT13900.030.77598846
TGCTTGA13550.029.7895955
GCTTGAA13800.029.4990556
CTCGTAT14450.029.1270939
ACGTATG3400.028.41253369
TCGTATG15350.027.4193140
CTTGAAA14800.027.2727757
TCTTCTG15200.027.23677350
CTTCTGC15050.026.82052651
CTGCTTG16000.025.44368454
TTCTGCT16500.024.88175452
TATGCCG17150.024.34032243
ATCTTTT42100.023.52033612
TCTTTTT44000.022.583113
Produced by FastQC (version 0.11.2)