FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392727
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGC703.8510054E-529.572093
CTAGATG2650.027.33925469
TTTCGGA4600.024.7638911
TATAAGG1401.3880708E-824.6434082
GTCTTCT3500.024.64252549
ATCTTTT21550.024.17455512
ATTATAT1902.7284841E-1123.6057933
TCTTTTT22950.023.00051513
TAAGGCA750.001992974423.0005134
TTACACC3000.023.0005134
TGCCGTC3900.022.99969145
AGTCTAC1202.4587498E-622.9996969
GATCTTT24550.022.6257611
ACGAATG13450.022.31568345
ATCTGGC9750.022.2920067
CTTTTTT23450.022.21584914
ATTAAGG800.002899361321.5629833
GAATGGC13550.021.38716547
GTATGCC4050.021.2960142
TAACGAC8150.021.1653655
Produced by FastQC (version 0.11.2)