FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C3_P2CW_und_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C3_P2CW_und_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926072
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG35460.18410526709281896No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT29490.15310954107634606No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA29120.15118853293127152No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA26530.13774147591574976No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT23080.11982937294140612No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG22310.11583159923408885No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA21030.10918594943491208No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA20120.10446130778080985No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT19910.10337100586063241No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT19910.10337100586063241No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA19550.10150191685461396No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT19480.10113848288122147No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA19440.1009308063249972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5600.0102.3050161
GCAAAAG36150.094.660223
GCAGGGT18600.071.3299569
AGCGAAA12000.070.102681
AGCAGGC4900.066.5809868
GCAGGGG13850.066.47959
GCGTGAC451.19419536E-464.44382145
AGCAAAA55500.062.181192
AGCAGGG36850.060.7931678
AAGCAGG65500.060.4345327
ACAAGGG9550.059.2140853
CAAAAGC59950.057.564084
AGGGTGT8550.054.268486
CAAGGGT10400.053.6773644
GAGCAAA64950.053.25951
TCGTACT5600.050.4918982
GCCTGCG604.946465E-448.332867145
TAACGAC450.00964085148.332863145
AAGGGTG9700.047.834595
TTAGTAG1602.0008883E-1145.3132362
Produced by FastQC (version 0.11.5)