FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C3_P2CW_und_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C3_P2CW_und_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803245
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG35810.19858643722844094No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA30100.16692130021156304No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT29070.1612093753206026No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA27130.15045099251626928No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG23600.1308751722588999No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT21220.11767674387007866No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT20810.11540306502998761No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA19830.10996841804635532No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA19470.10797201711359244No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT18950.10508832687737939No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA18430.10220463664116633No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA18190.10087370268599108No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT18120.10048551361573163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6300.0108.186681
GCAAAAG31400.097.6645663
GCAGGGT17750.077.19519
AGCGAAA11650.069.707351
GCAGGGG11700.069.399839
TTAGTAG1500.062.831642
AAGCAGG60450.061.6443257
AGCAGGG35450.061.3523648
ACAAGGG9950.061.204383
AGCAAAA51750.059.9597362
CAAGGGT10650.056.500824
CAAAAGC55400.056.1401824
ATTAGTA1700.055.447371
AGGGTGT8050.054.9364056
GAGCAAA56650.054.3970761
AGCAGGC3550.053.097168
AAGGGTG9000.050.7486345
GGTCGTT2550.048.332037
GTCGTTT2550.048.332038
CTACTAA1203.6252459E-948.3320276
Produced by FastQC (version 0.11.5)