FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C3_P2CW_100d_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C3_P2CW_100d_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722321
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG43680.2536112606186652No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA28610.1661130532577841No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT26110.1515977567480162No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA24580.14271439528403823No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG24540.14248215053988192No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT23320.13539868584311518No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT22710.1318569534947318No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA21620.125528284216473No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA20440.11867706426386254No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC19310.11211615024144744No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA18300.1062519704515012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5650.0106.514441
GCAAAAG34150.097.867533
GCAGGGT20900.081.514989
AGCAGGG33000.073.594738
AGCGAAA10650.072.847231
AGCAAAA49550.067.4505842
GCAGGGG10400.066.919669
AAGCAGG61550.063.8410037
ACAAGGG11600.060.6236653
AGGGTGT9250.059.5662546
CAAAAGC56250.059.5453534
CAAGGGT12100.057.5193944
GAGCAAA59400.056.760391
AAGGGTG10500.053.8559575
CTATTCG3700.052.9027029
TAAACTA4150.052.408485
AGCAGGC5250.051.092638
GGATACG752.5512523E-548.33367145
CTACTAA1501.0913936E-1148.332276
AAAGCAG96000.041.913146
Produced by FastQC (version 0.11.5)