FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C3_P2CW_100d_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C3_P2CW_100d_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006414
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG38270.1907383022646373No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA26790.13352179560150598No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA26740.13327259478851325No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT25380.12649433267511093No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT23900.11911798861052604No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG23060.11493141495224815No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA22260.11094420194436441No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT22190.1105953208061746No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA22070.10999723885499203No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC21700.10815315283884581No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA20870.10401641934316647No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA20450.10192313251402751No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA20220.10077680877426094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5900.0108.1510241
GCAAAAG30850.095.647643
GCAGGGT19150.073.8245549
AGCGAAA11850.071.592491
GCAGGGG10150.069.999529
AGCAGGG32450.065.46188
AAGCAGG60600.058.980627
AGCAAAA50650.058.8298032
TAAACTA6150.055.4061245
AGGGTGT11150.055.2686776
ACAAGGG13550.055.1103253
AGCAGGC3950.055.0629128
CAAAAGC55800.053.6600274
TTAGTAG2050.053.0563471
CTATTCG6300.052.9361429
CAAGGGT14250.050.8768424
AAGGGTG12450.049.497655
AGGTAGT5250.044.1901746
GAGCAAA69100.043.2333761
CTACTAA1557.403287E-1042.0964856
Produced by FastQC (version 0.11.5)