FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C2_P2CW_und_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C2_P2CW_und_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615282
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG30340.18783097935840307No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT25560.15823862334874036No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA22720.14065655408776917No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA19720.12208394571350391No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA18640.11539780669876838No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT18040.11168328502391534No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT18030.11162137632933443No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC17840.11044511113229764No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA17680.1094545720190035No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG17320.10722585901409165No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA17210.10654486337370193No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA16370.10134453302890764No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT16290.10084926347226059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4600.0110.3381961
GCAAAAG29450.089.115893
GCAGGGG13150.074.4286049
AGCGAAA9200.074.084221
GCAGGGT14750.063.89749
AGCAAAA42800.063.011182
AGCAGGC2850.061.051778
AGCAGGG31950.059.9052628
AAGCAGG53450.058.053447
CAAAAGC50350.052.5563624
GAGCAAA51350.052.2452661
ACAAGGG8850.051.6094443
TCGTACT4600.048.8564952
CTACTAA1650.048.3326536
CAAGGGT9450.047.5654684
AGGGTGT7700.047.0772636
GTCGTTT1751.8189894E-1245.570798
ACTAATA1751.8189894E-1245.570798
GGTCGTT1801.8189894E-1244.304937
TAAACTA3150.043.7295465
Produced by FastQC (version 0.11.5)