FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C2_P2CW_und_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C2_P2CW_und_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902622
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG43000.22600390408604545No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT35660.18742556324903212No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA33510.17612536804472986No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG28280.1486369862221713No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT25960.1364432872110172No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA22600.11878344726382856No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT21840.11478895965672635No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT21740.11426336918210764No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA20960.1101637634800817No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA20780.10921770062576801No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT19470.10233246540826291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6550.0115.14741
GCAAAAG37100.098.6856463
AGCGAAA13450.075.4862441
GCAGGGT21500.075.197419
GCAGGGG14650.069.2830359
AGCAAAA54050.068.006242
AGCAGGG40300.065.483648
AAGCAGG68800.063.4372867
CTACTAA1550.060.806246
AGCAGGC5900.058.9828458
GAGCAAA62450.058.8759041
CAAAAGC62850.058.714994
ACAAGGG10900.056.5365033
TCGTACT6600.054.9240532
TAAACTA4100.053.04865
CAAGGGT12350.050.4856574
AGGGTGT9700.050.0771456
CTATTCG4200.050.0593539
AAGGGTG10950.045.022685
TCTACTA2100.044.88085
Produced by FastQC (version 0.11.5)