FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C2_P2CW_100d_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C2_P2CW_100d_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210733
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG42770.1934652443329882No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA31630.14307471775198544No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT29880.1351587912244491No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT28320.12810230814847384No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA28100.12710716309929782No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC26530.12000544615745094No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA25270.11430597905762478No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG24030.1086969796895419No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA23910.10815417329908224No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT23890.10806370556733896No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA23340.10557584294439899No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT22830.10326891578494553No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA22530.10191189980879646No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT22410.1013690934183368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5250.0118.767031
GCAAAAG28950.090.9044953
AGCGAAA13350.082.5503851
ACAAGGG14800.065.6402743
GCAGGGT19800.065.1751569
TAAACTA4150.064.6369555
CAAGGGT16100.060.340134
CTATTCG4500.059.6096429
AGGGTGT13400.058.4313966
GCAGGGG11950.058.241249
AGCAGGG35500.056.977478
AGCAAAA48000.055.5819632
AAGGGTG14950.052.858235
AGCAGGC5150.052.0860988
AAGCAGG64050.050.9355137
CAAAAGC56700.047.309244
CAAGGTA5450.043.8980039
TCGTACT7700.039.5444832
TTTAAAC7050.039.077053
GAGCAAA70450.038.5928651
Produced by FastQC (version 0.11.5)