FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C2_P2CW_100d_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C2_P2CW_100d_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008540
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG54200.2698477501070429No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA36770.18306829836597727No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG31490.15678054706403657No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT29190.14532944327720634No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT29170.1452298684616687No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT28640.14259113584992084No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA27320.13601919802443566No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA25930.1290987483445687No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA24860.12377149571330419No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC23660.11779700678104493No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA21870.10888506079042488No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT21500.10704292670297827No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT21090.10500164298445638No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG21050.10480249335338106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6750.0112.803421
GCAAAAG39000.096.10693
AGCGAAA13750.074.8897551
GCAGGGT25500.072.214129
GCAGGGG12950.070.539019
AGCAGGC6100.068.932948
AGCAAAA54900.068.010242
AGCAGGG40950.066.567558
AAGCAGG72950.061.9143567
CAAAAGC65550.057.6227074
CCTCGCG400.00605228854.373825145
CTACTAA1750.053.8559766
GAGCAAA72900.053.2185441
TAAACTA5150.052.086255
ACAAGGG12750.051.7439773
TACTAAT2150.050.58037
ATTAGTA1900.049.6165431
CTATTCG5600.047.900759
CAAGGGT14000.047.123984
TCGTACT7100.046.9719852
Produced by FastQC (version 0.11.5)