FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C1_P2CW_und_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C1_P2CW_und_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848311
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG46030.24903817593467767No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA34620.1873061405791558No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT33550.18151707153179308No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG26760.1447808296331083No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT23770.12860389836991717No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT22750.12308534656775835No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA22000.11902758788970037No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA21550.1165929326828656No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC20930.11323851884233768No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA20780.11242696710672608No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT20710.11204824296344067No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT20510.11096617398262522No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT20280.10972179465468744No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA19210.10393272560732475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5950.0115.7719651
GCAAAAG38900.093.185143
GCAGGGT21350.077.761369
GCAGGGG15150.074.172919
AGCAGGG40000.069.779368
AGCGAAA11700.067.551871
AGCAAAA54500.067.311932
AAGCAGG67800.064.1575557
CAAAAGC61800.059.241934
GAGCAAA64350.057.5796621
ACAAGGG9550.052.380783
TAAACTA3700.050.944575
CTATTCG3800.049.6039249
CAAGGGT10300.048.5666474
AGGGTGT8500.047.7634126
TTAGTAG1307.346898E-944.6214181
AGCAGGC4250.044.351748
AAGGGTG9550.043.271085
AAAGCAG105400.041.5453646
TCGTACT5050.040.1980172
Produced by FastQC (version 0.11.5)