FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C1_P2CW_und_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C1_P2CW_und_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338559
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG46360.1982417377538903No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT38610.16510167158493755No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA38350.16398987581668883No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG30860.1319616054159848No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT30350.1297807752551892No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA27040.11562675989786873No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT26290.11241965672022815No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA25420.10869941703416505No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA24860.10630477999486007No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT24430.10446604083967946No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA24390.10429499533687198No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC23400.1000616191423864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6550.0114.043181
GCAAAAG43050.093.127243
GCAGGGG18250.070.3129359
AGCGAAA13800.069.369451
GCAGGGT25550.067.248459
AGCAAAA61350.065.702972
AGCAGGG48750.061.7160768
AAGCAGG79700.059.217157
CAAAAGC72150.056.0689854
CTATTCG4700.053.987729
GAGCAAA78200.052.2125661
TAAACTA5150.050.6780745
ACAAGGG14050.048.5038643
CTACTAA1650.048.3318636
AGGGTGT11950.046.7140586
CAAGGGT14750.045.7104724
TCGTACT7650.045.488812
AGCAGGC5500.044.8168228
AAGGGTG13400.042.7412385
CAAGGTA6850.039.1593934
Produced by FastQC (version 0.11.5)