FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_vTC35C1_P2CW_100d_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_vTC35C1_P2CW_100d_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021991
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG51070.2525728353884859No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA36910.18254285009181545No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG29780.1472805764219524No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT29480.1457968902927857No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT28290.13991160198042424No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA28200.13946649614167422No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT27710.13704314213070187No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC26200.12957525528056257No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA25910.12814102535570138No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA25780.12749809469972914No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT24300.1201785764625065No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA22620.11186993413917273No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT21410.10588573341820018No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG21230.10499552174070012No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT20860.10316564218139448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6000.0112.390591
GCAAAAG37900.095.454223
CTACTAA1450.079.999226
AGCGAAA12150.076.38921
GCAGGGT22950.075.816259
AGCAAAA53350.068.218642
AGCAGGG38300.068.1455468
GCAGGGG12850.067.703629
AAGCAGG69700.061.057517
TCTACTA2050.060.121375
AGCAGGC5950.059.70538
CAAAAGC62850.057.9071584
AGGGTGT11050.057.0808946
TAAACTA5000.056.549455
CAAGGGT13600.055.4406364
ACAAGGG13400.055.186033
TACTAAT2250.054.7772457
TCGCTCG400.0060520154.37447145
AAGGGTG11950.053.38865
ACTAATA2450.053.264798
Produced by FastQC (version 0.11.5)