FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38555678
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28488497.388922067457872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA72150.045.770676
CGGAGAT78500.044.275829
TCGGAGA84850.040.0215648
CTCGGAG90450.036.2643747
GGCTCGG156250.019.5168085
TGGGCTC153850.016.7801973
GGGCTCG158200.015.7984574
TATACTG148250.014.3647935
CTAGACT76650.012.9482314
TAGGACT82400.012.9238574
CGTGGGC141250.012.5984571
GTACTAG49650.012.3896661
TCTAGAC74900.012.1992543
GTAGGAC56850.012.1181893
CTATACT113200.011.8393354
TACACTG114800.011.6728415
TACACCG44200.011.6354415
TGCACTG127900.011.3834075
GTATTAG123500.011.0885361
CTAGGAC55000.011.0754433
Produced by FastQC (version 0.11.5)