FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_RNA_MRTPCR_NC_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_RNA_MRTPCR_NC_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6877
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGAC731.0615093790897194No Hit
AGCGAAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCG420.6107314235858659No Hit
GGCTTATAGTGCGGAGTGCCCATTCAACGAGGAGAGCGTTCATGAAACTG410.5961901992147739No Hit
ATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTC370.5380253017304057No Hit
GCCCTGGACACTGCTTCGAAACGCGAGCATCTCGCCGTGGCTGACGGCTT360.5234840773593137No Hit
CTATAAGCCAACGCGGTGCTGCCGGCGGTAACGGTTTGTCAGGTGCGCCG360.5234840773593137No Hit
GTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGAATCCGGCATCCTCGA220.319906936164025No Hit
TCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGA220.319906936164025No Hit
AAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTT210.3053657117929329No Hit
CTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATCGCCACGTA190.2762832630507489No Hit
GTCCAGGGCTCTGCGGGCTACGTGGCGATGGAAACCGTCCAGGGTGCGCT190.2762832630507489No Hit
AGCGAAAGCAGGTTGACCACCGTCTGCGCAGTGCTGTTCAACCTGCTGGT180.26174203867965684No Hit
GGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATC180.26174203867965684No Hit
AAAGCAGGTTGACCACCGTCTGCGCAGTGCTGTTCAACCTGCTGGTCGAC170.24720081430856478No Hit
GGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGAATCCG170.24720081430856478No Hit
AAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATCGCCAC160.23265958993747274No Hit
GCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATCGCCACGTAGC160.23265958993747274No Hit
ACATGCGGCCAAGCCCGCTGCTTGCCGCGACGGCGCTCAGGGATTCCGGA160.23265958993747274No Hit
GGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAG150.21811836556638067No Hit
ATAGTGCGGAGTGCCCATTCAACGAGGAGAGCGTTCATGAAACTGCAGGC140.20357714119528864No Hit
AAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTAT140.20357714119528864No Hit
GGTCGAATCCGGCATCCTCGATGCCGGCGCACCTGACAAACCGTTACCGC140.20357714119528864No Hit
GACAAACCGTTACCGCCGGCAGCACCGCGTTGGCTTATAGTGCGGAGTGC140.20357714119528864No Hit
TCCCTGAGCGCCGTCGCGGCAAGCAGCGGGCTTGGCCGCATGTCGCTCGA130.1890359168241966No Hit
CCCTGGACGGTTTCCATCGCCACGTAGCCCGCAGAGCCCTGGACACTGCT130.1890359168241966No Hit
AGCGAAAGCAGGGCAGGGGGAGAGGGGCTGGCCTCAAAGAGAGAGAGAGA130.1890359168241966No Hit
AGCGAAAGCAGGGTCAAGGTTCAGAGGTCAAAGGTCACAGGTCAAGGGTC130.1890359168241966No Hit
TTATCCCTCAGGTCGAATCCGGCATCCTCGATGCCGGCGCACCTGACAAA120.17449469245310456No Hit
GTACGGGATCGCGTACATCATGTCGACGACGCGCATCATCACGGAGTCGA120.17449469245310456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.1599534680820125No Hit
GAGCGAAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGC100.14541224371092046No Hit
CGTCTGCGCAGTGCTGTTCAACCTGCTGGTCGACCTCGCGTATGCGTGGC100.14541224371092046No Hit
AGTGCGGAGTGCCCATTCAACGAGGAGAGCGTTCATGAAACTGCAGGCCA100.14541224371092046No Hit
AGCGAAAGCAGGGGGTCGATGAATGGTGATATTTACACAGTAATGTAAGT100.14541224371092046No Hit
AGCGAAAGCAGGAGAGAGGACATCTAAATGCACAGTCCAATACAAGGCTG100.14541224371092046No Hit
CCTGACAAACCGTTACCGCCGGCAGCACCGCGTTGGCTTATAGTGCGGAG100.14541224371092046No Hit
ATTATCCCTCAGGTCGAATCCGGCATCCTCGATGCCGGCGCACCTGACAA100.14541224371092046No Hit
GGCTGACGGCTTCCAGGTCGCCGTGAAATTGCTTGTCGAGCGACATGCGG100.14541224371092046No Hit
CCCTCAGGTCGAATCCGGCATCCTCGATGCCGGCGCACCTGACAAACCGT100.14541224371092046No Hit
GGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGG100.14541224371092046No Hit
CCGCTGGCCTGCAGTTTCATGAACGCTCTCCTCGTTGAATGGGCACTCCG100.14541224371092046No Hit
AAGCAGGAGAGAGGACATCTAAATGCACAGTCCAATACAAGGCTGACCAC90.13087101933982842No Hit
ACTGTGTGTTGCAGGCCTGCTAGTTACCTCTGGCCCACTAGCTCAGAGCC90.13087101933982842No Hit
CGTTGAATGGGCACTCCGCACTATAAGCCAACGCGGTGCTGCCGGCGGTA90.13087101933982842No Hit
GCCACGTAGCCCGCAGAGCCCTGGACACTGCTTCGAAACGCGAGCATCTC90.13087101933982842No Hit
GTTGACCACCGTCTGCGCAGTGCTGTTCAACCTGCTGGTCGACCTCGCGT90.13087101933982842No Hit
AGCCCGCTGCTTGCCGCGACGGCGCTCAGGGATTCCGGATTCAGCTTCAC90.13087101933982842No Hit
TCACTGTGTGTTGCAGGCCTGCTAGTTACCTCTGGCCCACTAGCTCAGAG90.13087101933982842No Hit
GCGAAAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACG90.13087101933982842No Hit
CTAATAATGTTGTGAATTTACAGTGGGCGCATGCCTTGTTCTTTCTCTCT90.13087101933982842No Hit
TTCCATCGCCACGTAGCCCGCAGAGCCCTGGACACTGCTTCGAAACGCGA90.13087101933982842No Hit
ATACAAGGCTGACCACTGGGGTAATTACCAAAGCAGCCTCTCTTCCCCAC90.13087101933982842No Hit
AAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTA90.13087101933982842No Hit
CTAAATGCACAGTCCAATACAAGGCTGACCACTGGGGTAATTACCAAAGC90.13087101933982842No Hit
GCTTCGAAACGCGAGCATCTCGCCGTGGCTGACGGCTTCCAGGTCGCCGT90.13087101933982842No Hit
GGTTGACCACCGTCTGCGCAGTGCTGTTCAACCTGCTGGTCGACCTCGCG80.11632979496873637No Hit
TGCTTGTCGAGCGACATGCGGCCAAGCCCGCTGCTTGCCGCGACGGCGCT80.11632979496873637No Hit
CCAGCGGTCCGTTCGAAGTGAAGCTGAATCCGGAATCCCTGAGCGCCGTC80.11632979496873637No Hit
AAAGCAGGGGGTCGATGAATGGTGATATTTACACAGTAATGTAAGTAACT80.11632979496873637No Hit
GTGTGGGGGTGGCTCAGGAGTGTGACCCAGTCTCTTCAGAGGTATGGCCC80.11632979496873637No Hit
GTGCTGCCGGCGGTAACGGTTTGTCAGGTGCGCCGGCATCGAGGATGCCG80.11632979496873637No Hit
TCGCAAGCGCGAGCGGCGAGCGCGAACGCGGCGGCGAATCGGTCTGCACG80.11632979496873637No Hit
TGCGTGGCTCGATCCGCGCATCCGTTATTGAGCGAGGCACCGCGATGACT80.11632979496873637No Hit
CATGAAACTGCAGGCCAGCGGTCCGTTCGAAGTGAAGCTGAATCCGGAAT80.11632979496873637No Hit
TCTAAATGCACAGTCCAATACAAGGCTGACCACTGGGGTAATTACCAAAG80.11632979496873637No Hit
CCGTTATTGAGCGAGGCACCGCGATGACTCCGACTACTCCCGTCGTCGGT80.11632979496873637No Hit
GCTTCCAGGTCGCCGTGAAATTGCTTGTCGAGCGACATGCGGCCAAGCCC80.11632979496873637No Hit
TTCCAGGTCGCCGTGAAATTGCTTGTCGAGCGACATGCGGCCAAGCCCGC80.11632979496873637No Hit
CTACCAGGACGTGCAATAAAGAACTTAAACAGGAGACAAGATGCATGCAG80.11632979496873637No Hit
GTTTCCATCGCCACGTAGCCCGCAGAGCCCTGGACACTGCTTCGAAACGC80.11632979496873637No Hit
GCTAGTTACCTCTGGCCCACTAGCTCAGAGCCCTGTGGCTGTTTGTTGAT80.11632979496873637No Hit
AGCGAAAGCAGGTACGGGATCGCGTACATCATGTCGACGACGCGCATCAT80.11632979496873637No Hit
GGCCAAGCCCGCTGCTTGCCGCGACGGCGCTCAGGGATTCCGGATTCAGC80.11632979496873637No Hit
GTGTGTTGCAGGCCTGCTAGTTACCTCTGGCCCACTAGCTCAGAGCCCTG70.10178857059764432No Hit
GGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTC70.10178857059764432No Hit
GCGAAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGA70.10178857059764432No Hit
GCAGGGGGTCGATGAATGGTGATATTTACACAGTAATGTAAGTAACTCCA70.10178857059764432No Hit
CACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGAA70.10178857059764432No Hit
ATCTTACCCTGTGTACACTGACAGAAGCACAAATGGGACTTACACTACCA70.10178857059764432No Hit
GAGAGAGAGAACATGATCAACAAACAGCCACAGGGCTCTGAGCTAGTGGG70.10178857059764432No Hit
CGCCAAGCGCACCCTGGACGGTTTCCATCGCCACGTAGCCCGCAGAGCCC70.10178857059764432No Hit
TTATAGTGCGGAGTGCCCATTCAACGAGGAGAGCGTTCATGAAACTGCAG70.10178857059764432No Hit
AAAGCAGGCAGAGAGCTAATAGCTGAAAACCCTTTTTTCTCCAGTACCTG70.10178857059764432No Hit
CAATAACGGATGCGCGGATCGAGCCACGCATACGCGAGGTCGACCAGCAG70.10178857059764432No Hit
TCATGAAACTGCAGGCCAGCGGTCCGTTCGAAGTGAAGCTGAATCCGGAA70.10178857059764432No Hit
AGCGTGACCAGCGCCAGCAGCACGAGGCTGAACACGGCCGCGCGGTTGTG70.10178857059764432No Hit
CAACGAGGAGAGCGTTCATGAAACTGCAGGCCAGCGGTCCGTTCGAAGTG70.10178857059764432No Hit
GCACATCACTGTGTGTTGCAGGCCTGCTAGTTACCTCTGGCCCACTAGCT70.10178857059764432No Hit
ACATTATAGTCTGCCATATGAGTAATTCAGTCTCTAATAATGTTGTGAAT70.10178857059764432No Hit
AGTTACCTCTGGCCCACTAGCTCAGAGCCCTGTGGCTGTTTGTTGATCAT70.10178857059764432No Hit
GTCGAGCGACATGCGGCCAAGCCCGCTGCTTGCCGCGACGGCGCTCAGGG70.10178857059764432No Hit
ATTCAACGAGGAGAGCGTTCATGAAACTGCAGGCCAGCGGTCCGTTCGAA70.10178857059764432No Hit
CTGCTTCGAAACGCGAGCATCTCGCCGTGGCTGACGGCTTCCAGGTCGCC70.10178857059764432No Hit
CCTCTGGCCCACTAGCTCAGAGCCCTGTGGCTGTTTGTTGATCATGTTCT70.10178857059764432No Hit
GTTCGAAGTGAAGCTGAATCCGGAATCCCTGAGCGCCGTCGCGGCAAGCA70.10178857059764432No Hit
CATTCAACGAGGAGAGCGTTCATGAAACTGCAGGCCAGCGGTCCGTTCGA70.10178857059764432No Hit
AAAGCAGGGTCAAGGTTCAGAGGTCAAAGGTCACAGGTCAAGGGTCAAAC70.10178857059764432No Hit
TGCGCACGCTGATCGGCGGCCGCGTGTCGATCGAGGTCGGCGTGCTCGGC70.10178857059764432No Hit
CGTATCAATATGCATTCTGAAAAGCCCTGAATCTGTTTAGGTGTCTGACT70.10178857059764432No Hit
ATCCTCGATGCCGGCGCACCTGACAAACCGTTACCGCCGGCAGCACCGCG70.10178857059764432No Hit
CGCCGTGGCTGACGGCTTCCAGGTCGCCGTGAAATTGCTTGTCGAGCGAC70.10178857059764432No Hit
TTCATGAACGCTCTCCTCGTTGAATGGGCACTCCGCACTATAAGCCAACG70.10178857059764432No Hit
TCAATATGCATTCTGAAAAGCCCTGAATCTGTTTAGGTGTCTGACTGGAG70.10178857059764432No Hit
AACGAGGAGAGCGTTCATGAAACTGCAGGCCAGCGGTCCGTTCGAAGTGA70.10178857059764432No Hit
CCGTTACCGCCGGCAGCACCGCGTTGGCTTATAGTGCGGAGTGCCCATTC70.10178857059764432No Hit
GGCCAGCGGTCCGTTCGAAGTGAAGCTGAATCCGGAATCCCTGAGCGCCG70.10178857059764432No Hit
TATCAATATGCATTCTGAAAAGCCCTGAATCTGTTTAGGTGTCTGACTGG70.10178857059764432No Hit
CCCTGAGCGCCGTCGCGGCAAGCAGCGGGCTTGGCCGCATGTCGCTCGAC70.10178857059764432No Hit
CAGCTTCACTTCGAACGGACCGCTGGCCTGCAGTTTCATGAACGCTCTCC70.10178857059764432No Hit
GGTCACGCTCGCGTGCTTCGTCGGCCCGTGGCTGCTCGCGGCCGATCCCG70.10178857059764432No Hit
GTTTGTCAGGTGCGCCGGCATCGAGGATGCCGGATTCGACCTGAGGGATA70.10178857059764432No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTA100.006938941145.03
GTGTATG100.006938941145.01
TATGTAT100.006938941145.04
AGGGTTT100.006938941145.0145
ATGTATG100.006938941145.05
AGCGAAA256.0141574E-6116.01
AGCAGGA203.702467E-4108.757
GCAGGAC258.990981E-487.08
GCGAAAG451.11154135E-464.444441
CGAAAGC451.11154135E-464.444442
GAAAGCA451.11154135E-464.444443
CAGGACG400.005798514554.3759
AAGCAGG656.836326E-444.6153835
AAAGCAG750.001382803438.6666644
AAAAAAA5855.280155E-68.675214145
TAAAAAA1900.00228017877.6315794145
GGGGGGG22050.07.5623584145
ATCATTA1750.0094407137.457143135-139
TTAAAAA1750.0094407137.457143140-144
Produced by FastQC (version 0.11.5)