FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_Plasmid_control_NA_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_Plasmid_control_NA_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543275
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC72910.4724368631643745No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT61770.40025270933566603No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG48810.31627545317587596No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT44830.2904861414848293No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG38130.24707197356271565No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG31590.20469456188948826No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG31020.20100111775283083No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC27550.17851646660510925No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC27530.17838687207399848No Hit
ATATAAGCTTTCGCAATTTTAGAAAAAAACTCCTTGTTTCTACTAATAAC27140.17585977871733813No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT26780.17352707715734397No Hit
AGCTTATATAGGCATGAGATTGATGTCCGCCCCATCAGGCCATGAGCCTG25980.16834329591291247No Hit
TACTAGCACATTGTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCCTGCT25740.16678816153958304No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA25730.16672336427402762No Hit
GTTTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT25270.16374269005847952No Hit
TTCATATCCTGAGCGTAACTTCTCGCTGATCGTTCTTCCCATCCACACGT24880.16121559670181917No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA24020.15564303186405534No Hit
ATCCTGACAAGTGTTATCAATTTGCCCTTGGACAGGGAACAACACTAAAC21810.14132283617631336No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC21720.14073966078631484No Hit
GTATTGTTTCATGGTCTAAAGAAATCCTCAGGACCCAGGAGTCAGAATGC21070.13652783852521425No Hit
GTAGTAATGACTGATGGGAGTGCTGATACTAAAATACTATTCATTGAGGA20880.13529669047966175No Hit
ATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTG20220.13102007095300577No Hit
TCATTGAGGAGGGGAAAATCGTTCATACTAGCACATTGTCAGGAAGTGCT20200.130890476421895No Hit
GACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGT19930.12914095025189937No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT19560.12674345142634982No Hit
CTTGATAACTACTGTAACATTGCATTTCAAGCAATATGAATTCAACTCCC19210.12447554713191104No Hit
GTCTAAAGAAATCCTCAGGACCCAGGAGTCAGAATGCGTTTGTATCAATG19040.12337399361746935No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG18590.12045811666747663No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT18260.11831980690414864No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG18190.1178662260452609No Hit
TCTTTAGACCATGAAACAATACTATCTACAAGCCTCCCATTGTAAATGAA18080.11715345612415157No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT18030.11682946979637458No Hit
CTACTGTAACATTGCATTTCAAGCAATATGAATTCAACTCCCCCCCAAAC17860.1157279162819329No Hit
ATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCA17710.114755957298602No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT17690.1146263627674912No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT17680.11456156550193582No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC17620.11417278190860346No Hit
ACACTAAACAACGTGCATTCAAATGACACAGTACATGATAGGACCCCTTA17560.1137839983152711No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT17550.1137192010497157No Hit
GTATTTTAGTATCAGCACTCCCATCAGTCATTACTACTGTACAAGTTCCA17380.11261764753527401No Hit
CCATAAGAGAAGAGGGACAGCTATGACTGGGAGTAGTCAGGAGAGGAGGA17200.11145129675527693No Hit
CTAAAATACTATTCATTGAGGAGGGGAAAATCGTTCATACTAGCACATTG16980.11002575691305826No Hit
GTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCCTGCTATCCTCGATATC16550.10723947449417634No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT16200.10497157019973757No Hit
TATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG16190.10490677293418219No Hit
CTCCATAAGAGAAGAGGGACAGCTATGACTGGGAGTAGTCAGGAGAGGAG16060.10406440848196206No Hit
ATACTATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTT15900.10302765223307578No Hit
GTGCTAGTATGAACGATTTTCCCCTCCTCAATGAATAGTATTTTAGTATC15770.10218528778085564No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA15690.1016669096564125No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT15640.10134292332863552No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC301.5996118E-596.66818145
TTTCGCG554.062271E-665.910126145
GTCCGTA701.6962613E-551.786526145
CTGATGA17500.044.3263939
TACTGTG30150.043.7623637
ACTGTGT30200.043.2097978
TACTGAT19900.042.987847
TATACTG20200.042.7082985
TATTGCG855.3423544E-542.647728145
AAGCCCG855.3423544E-542.647728145
ATACTGA20100.042.5600976
CTGTGTC31500.041.886839
CATATAC21200.040.0098273
ATGATAG29150.039.046115
GGAGACC1502.600791E-838.66727145
GTACTGT34550.038.1891486
GATAGGA28900.037.878767
TGATAGG29900.037.824236
ATAGGAC29000.037.7481468
TAGGACC29100.037.3692979
Produced by FastQC (version 0.11.5)