FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_No_index_control_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_No_index_control_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTGAGCAGGGAGACGTGTGCTCTTCCGATCTGAGCAGGGACTGTCTC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAGCGCACCC11.5151515151515151No Hit
GATCGGAAGAGCACACCCCCGGTTTAGATCGGAAGAGCACCTGTCTCTTA11.5151515151515151No Hit
GGTGTACTTCCGCCCCACGCCGGGAAGGATTGCTTCCTCTACACTCATGC11.5151515151515151No Hit
ACAATAAATTTAGAATCAGCAAAGGGAAACAAACAAACATTCAGTGATCA11.5151515151515151No Hit
ATCCTTCATTACTCATGTCACAGGAATGCACGATCGGGCTCGCGGCCTTT11.5151515151515151No Hit
CTCCCAGGCCGGTGCAAGCTGTTGCGGTGGGTGCCACTGCGGCGGGCGCT11.5151515151515151No Hit
CTTCCGATCTCTGCCGGGCGTAACGAGCATCTTTACTCGTAATGCAATTT11.5151515151515151No Hit
AAAAAAATCAAAAAAAAAGAAGAACTGATATTAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
ATTATGCTGAGTTGTAACAGCACTGAAATATAATTTGCTGATGAGGGAAT11.5151515151515151No Hit
GGGTACGGGCGGACCCGCCACCTCGCTCACGAAGCTTTTCTCGACAGTAC11.5151515151515151No Hit
TTTTTACTCCTTGGGATCGCCGACTGTGTCATAGGTCACACATGCGGTCC11.5151515151515151No Hit
GAAAAGTATTAATATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
GTCGCCGAGCAGACTGAGGTTAGACTGCCGAGCAGATTGCCGGCCAACCC11.5151515151515151No Hit
AAAACCCGGAGACACAATAATATTTGAGGCAAATGGAAATCTAATAGCAC11.5151515151515151No Hit
TCTTCCGATCTCTTTAGGGAGACGTGTGCTCTTCCGATCTCTTTAGGGAG11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTCCCATAGAAGATCGGAAGAGCACACGTCTCCCATAGAAGATCGGAA11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGAGCTGAGGTGGGCATTGAGGATATACCCTGGGTGGGGGGCAAAA11.5151515151515151No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGATCAGGCACA11.5151515151515151No Hit
GACGTGTGCTCTTCCGATCTAGACCGGGAGACGTGTGCTCTTCCGATCTC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGCTGAGTGGTGTCCTTGCGGTGTAGAGGGGTGGGGTCATGTGTGGCCAG11.5151515151515151No Hit
GTCAACAGTGTCGGTTGAATTGTTCGCATGGTAGCCTATACATCTGTCTC11.5151515151515151No Hit
CGCATCGAGGCCATCAATTTCTCGATCGCCCACGACGACGGCCAGTCGGC11.5151515151515151No Hit
CTAGAAAGGGAGACGTGTGCTCTTCCGATCTGATTCTGTCTCTTATACAC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCAACTTAGATCGGAAGAGCACACGTCTGAAGAGCACACGTCTCCC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGCCGGTGGACTCGGCACGCGTCTGAGCGAGGAGACGGCGTTGCGGCCGA11.5151515151515151No Hit
CTATTCAACTTTTCAGAGGAAGAAAGAAAAACTAAGGATCACAGAGCTGT11.5151515151515151No Hit
GTCCCTAAACCAGATCATGGTCCGAGGTTAGAAGTCCAATTTCATCCTGT11.5151515151515151No Hit
GTGGAGGCCATTATCTTAAGGAAACAACTCAGAAACCCAAAGTCAAACAC11.5151515151515151No Hit
GTATTATGCGGCGCCCGCCTGAATCTGGGCCAAAAGACATACACCACTCC11.5151515151515151No Hit
CTCCGCCGTGATGTTCATGGCGCGAGGCTGGCGTCGAACTTCCCGGCCTG11.5151515151515151No Hit
GAAGAGCACCCTGTCCAGATCGGAAGAGCACCTGTCTCTTATACACATCT11.5151515151515151No Hit
GTGGTCGTCCGGTGGTTTTTTTTTTTTTTTTTTTTTTGTTTTTTGGGTTG11.5151515151515151No Hit
AAAATAGGGTGCTCTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
ACTCTGCATTCAATAATCTATAAGGAACTGAAAAGGCTAACACATATTTC11.5151515151515151No Hit
CCATTGCGGCGGGTAGAGGAACAGCGGCTGCGCGCGCACATAGGCCTGCG11.5151515151515151No Hit
CTTATAAGCAGTGCCATAGGCCAGGTTTCAAGGGACAGGTTCTTGTAATG11.5151515151515151No Hit
CTTCCGCTCTTTTTTTTTTTAAAAAAAAAAAAATAAAAAAAAAAAAAAAA11.5151515151515151No Hit
ACCGTCTTCCTGATGGCACGCTCGGGGGTCAGCGCGATCTGCGTGGATCG11.5151515151515151No Hit
GGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAACC11.5151515151515151No Hit
GGTGTTATTGTATGGCCAAACCCAAAAGCGTCAGCGGACAGGGGGAACCA11.5151515151515151No Hit
GATCTATCTCGCGCATCGCACCGAAGGGGCGATCCAGCAACGTGCGACGA11.5151515151515151No Hit
GACGTGTGCTCTTCCGATCTCAAAAGGGAGACGTGTGCTCTTCCGATATC11.5151515151515151No Hit
GGTCGGAAGAGCACACGTCTCCCAATTCAGATCGGAAGAGCACACGTCTC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCCGGGGAGATCGGAAGAGCACACGTCTCCGGGTCCTGTCTCTTATACA11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCATACGTCTCCCTCGCCAGATCGGAAGAGCACACGCCTCCCTCTAGAGC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGGGACGTGTGCTCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
CTTAGTATCATAAACCACAAAGCGATCAAGTATAAGAAAAAAAAACAGAA11.5151515151515151No Hit
CACATAAGCGGGCTCGCGCTCGAACACCTGCATGATGCCTCCGGCCACGG11.5151515151515151No Hit
GTGCTCTTCCGATCTGACAGGGGTGTGTGTTCTCTGTCTCATATACACAT11.5151515151515151No Hit
CACCCTCCCCAGATCGGAAGAGCACACGTCTCCCATGGGAGATCGGAAGA11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTTTTTCGAGTTTTACAAAGCGCCGGAGTTGATCGCGCTGGGCCGGGAG11.5151515151515151No Hit
TTAGTAGACTCTTTCTCCGCTGACTGAACATCTGGGGCAGTCTACAGCCC11.5151515151515151No Hit
AGGGAGACGTGAGCTCTTCCGATCTTAGTAGCTGTCTCTTATACACATCT11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATAGGCAAGGAGCGAAAGGGTTAGCCATCTCAGGAAGAAGACGTAGCGA11.5151515151515151No Hit
CCCATCTCCCTCACGGCGCCCGCGCCCTCGCCAGTGCCGCCGGCGGCGTC11.5151515151515151No Hit
GTGCAAGGGCCTGGCAGGCCGTCGCGCTGTGGGTCCCTGTCCTCCCGGCT11.5151515151515151No Hit
CTCGTGAGGAGGCGGAAGGTTTCGAGCCTGGTGCGGTCATTACCGGTGAG11.5151515151515151No Hit
CTGGGAGACGTGTGCTCTCCCGATCTCCACTGGGAGACGTGTGCTCCTGT11.5151515151515151No Hit
AGAAAAGTGAGATCTTCGAACTCAGCATTCCCTGGGTTCCGGCTCTCTCT11.5151515151515151No Hit
TCTGGGCTGTTTCCCTCTCGACGACGAAGCTTATCCCCCGCCGTCTCACT11.5151515151515151No Hit
CTTCCGATCTGAACAGGGTGTGCTCTTCCTAAAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
CTTCCGATCTAGGCTGGGATGGCTTCTCCGGTATGACGATTTTCAAGGAG11.5151515151515151No Hit
ACGTTATGATGCGAGGTTAAGTTGAAGAGACGGAGCCGTAGGGAAACCGA11.5151515151515151No Hit
CTTCCGATCTGGCTCGGGAGACGTGTCCTCTTCCAAAAAAAAAAAAAAAA11.5151515151515151No Hit
CTTCCGATCTGGGCGGGGGGTACGATACCCGGTTGCCCGGGCGGACGCCC11.5151515151515151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCT50.0145.08
CACATAA50.0145.01
ATAAGCG50.0145.04
TAAGCGG50.0145.05
GTGTAGA50.0145.0145
CGGGCTC50.0145.09
ACATAAG50.0145.02
AGCGGGC50.0145.07
AAGCGGG50.0145.06
CATAAGC50.0145.03