FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_9424.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_9424.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14559174
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173900.11944358931351463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA77650.086.830226
CGGAGAT89300.079.399339
TCGGAGA88900.078.696428
CTCGGAG87600.076.802147
GGGCTCG70850.072.345084
GGCTCGG85600.070.296515
CGTGGGC77250.046.5577161
TGGGCTC164200.031.8340243
GTGGGCT117550.031.7008862
CTACACT87250.017.117144
TACACTG115500.016.822185
GATCGGA19950.015.6262576
TATACTG165150.015.2767195
TACACCG37300.014.3830935
TATACCG27550.014.2102465
GTATTAG107000.014.1635121
CCTACAC71900.013.9148863
TGGCTCG43800.013.7383134
CTATACT109500.013.572794
TCCGAGA29450.013.539638
Produced by FastQC (version 0.11.5)