FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_8724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_8724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13934668
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA51750.078.5933156
CGGAGAT64850.066.294799
TCGGAGA64200.066.17558
GGCTCGG56550.063.9736375
CTCGGAG64600.063.408967
GGGCTCG49750.061.933754
CGTGGGC56100.041.101841
GTGGGCT110300.021.3618762
TGGGCTC156100.020.713983
TATACTG152750.015.5677465
CTATACT107200.015.5548074
GTGTAGG68700.014.6708431
CCTATAC56350.014.667043
TATACCG21850.014.2675735
ATACCGT33700.013.9835216
TACACTG115100.013.9203475
CTACACT91300.013.4992514
TCTACAC94600.013.4115343
CTTACAC86250.013.2809633
GTATAGG58650.013.2285661
Produced by FastQC (version 0.11.5)