FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_8724.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_8724.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13665259
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA66700.076.5209356
CGGAGAT79500.069.76299
TCGGAGA80150.067.9307868
GGCTCGG70850.064.978145
CTCGGAG87200.060.277467
GGGCTCG66000.057.0109674
CGTGGGC72800.038.049141
TGGGCTC129100.029.595073
GTGGGCT98900.029.5428032
GTATAGG55850.015.7099591
CTATACT87150.015.4732154
CTACACT74900.014.9064394
TATACTG112950.014.8913875
TACACTG91200.014.0705765
GTCCTAG46050.013.85689451
GTGTAGG68550.013.6456781
TACACCG39900.013.4459725
GTATAGA94950.013.3645991
CCTATAC53050.013.2562693
TGGCTCG49700.012.9828074
Produced by FastQC (version 0.11.5)