FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_8721.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_8721.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11720783
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA36600.065.3672946
CGGAGAT42400.061.3841489
TCGGAGA43200.058.2335668
CTCGGAG44200.055.1118057
GGGCTCG36950.049.4438064
GGCTCGG44500.047.8977365
CGTGGGC46000.029.0038851
TATACTG128500.015.797295
TCTATAC86500.015.4215323
GTGTAGG61700.015.3950951
GTGGGCT91550.015.3628892
CTATACT92850.015.1476624
CTACACT73100.015.0748164
GTATTAG89500.014.9880421
TAGACTG78000.014.7784945
TACACTG100850.014.7368825
TGGGCTC135000.014.4995933
GTGTAAG73800.014.3447411
TCTACAC73050.014.0926913
GTATAGG48800.013.9670351
Produced by FastQC (version 0.11.5)