FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_8404.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_8404.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13705941
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158710.11579650021840894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA66150.086.47286
TCGGAGA76500.078.374528
CGGAGAT78500.077.116849
CTCGGAG79750.072.180627
GGCTCGG72200.069.1853945
GGGCTCG62450.068.2614754
CGTGGGC68150.045.4314041
GTGGGCT112900.028.2550352
TGGGCTC159300.027.4430483
TACACTG103600.015.7454535
TATACTG137650.015.3793865
GTATTAG99350.014.888691
CTACACT84750.014.713624
TACACCG29950.014.5240195
TATACCG24000.014.4998125
GTATAGG58900.013.6647541
GTATAGC55600.013.4324931
TGGCTCG40050.013.3955324
CTATACT96650.013.2770694
GTGTAAG83700.013.2544021
Produced by FastQC (version 0.11.5)