FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_7379.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_7379.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19169364
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA105800.047.6246346
CGGAGAT131350.041.3965039
GGCTCGG117850.038.3257755
GGGCTCG103700.038.1720284
TCGGAGA138650.037.8574378
CTCGGAG136200.037.1013157
TGGGCTC145250.027.9014643
GTGGGCT130500.022.0553342
CGTGGGC131850.022.052621
TACACTG110450.015.7535345
TATACTG181800.015.472855
CTATACT169550.014.8803394
TACACCG79550.014.8552485
CTTACAC100150.014.6952323
TCTATAC168850.014.4697233
GTATAGG110350.014.1273941
GTATAGA178700.013.7147571
TATACAG194850.013.6179965
CTACACT112850.013.5553984
TATACCG69950.013.2664515
Produced by FastQC (version 0.11.5)