FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_6331.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_6331.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16491857
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214690.130179397019996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA77900.094.648336
CGGAGAT89600.086.982119
TCGGAGA88600.086.0818258
GGCTCGG83150.080.9124155
GGGCTCG73050.079.79314
CTCGGAG94150.079.159367
CGTGGGC74000.057.0290071
GTGGGCT140100.030.7899952
TGGGCTC202600.029.521973
CGGCTCG22100.016.0743454
TATACTG180550.015.49955755
TGGCTCG41450.015.3917364
TACACTG143100.014.7935595
CTATACT125300.014.2914194
GTGTAGG81200.014.0200041
GTATAGA131100.013.9933721
GATCGGA17850.013.8092546
CCTACAC76450.013.750593
TAGACTG108750.013.5330685
TATACAG168400.013.21678455
Produced by FastQC (version 0.11.5)