FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_6119.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_6119.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12699065
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136310.10733861114971849No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA42600.074.881356
CGGAGAT46600.072.498769
TCGGAGA46600.071.098578
CTCGGAG50250.063.1929477
GGCTCGG45150.063.1052365
GGGCTCG41300.060.9125254
CGTGGGC45250.039.1007961
GTGGGCT100500.019.4771232
TGGGCTC140300.018.602533
TACGCGG16850.016.78026145
TATACTG153050.015.9160955
CTATACT95250.014.5376944
GTATTAG92450.014.3535231
TACACTG110250.013.7435285
GTGTAGG61200.013.5072761
CTACACT79450.013.4137614
GTAGGAC38450.013.3871823
CTAGGAC55400.013.3480423
CTAGACT67050.013.1913184
TCTATAC95550.012.9745583
Produced by FastQC (version 0.11.5)