FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_5764.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_5764.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20281227
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA67100.080.493566
TCGGAGA78450.072.544438
CGGAGAT85550.068.218679
CTCGGAG83250.065.052477
GGCTCGG74050.063.93165
GGGCTCG62950.063.1120454
CGTGGGC72750.040.864761
GTGGGCT152300.019.7076242
TGGGCTC229900.018.1640663
TATACTG246250.017.3406935
CTATACT155950.015.2481054
TACACTG178650.014.9744455
TACACCG29950.014.5238525
CTACACT134050.014.4401554
CCTATAC82450.014.4205813
GTGTAGG98250.014.3175021
TATACCG25100.013.86420255
TCTATAC153600.013.8294843
TGGCTCG44050.013.8248624
GATCGGA18850.013.4612096
Produced by FastQC (version 0.11.5)