FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_5764.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_5764.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20572212
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA102400.071.224036
CGGAGAT123900.063.6043559
TCGGAGA125550.061.786798
CTCGGAG121150.061.278087
GGCTCGG108350.060.755195
GGGCTCG95350.059.23024
CGTGGGC113400.035.8717271
TGGGCTC188150.030.7100523
GTGGGCT151800.027.3660072
TATACTG208850.016.3498175
TACACCG67750.015.9442245
CTATACT155850.015.9091054
CTACACT118150.015.831154
TATACCG51200.014.8677675
TACACTG142900.014.3575615
CCTATAC91950.014.0344293
TACACAG171550.014.0305215
GTATAGG95950.013.980711
CCTACAC104850.013.8980563
TCTACAC144200.013.6750873
Produced by FastQC (version 0.11.5)