FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_4340.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_4340.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17965805
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA67350.095.157766
TCGGAGA76300.088.461088
CGGAGAT79850.084.9822169
GGCTCGG74950.075.5455555
GGGCTCG66100.074.691984
CTCGGAG90850.071.421287
CGTGGGC66250.050.6766661
GTGGGCT143150.024.4111182
TGGGCTC211650.023.7037663
GCCGACC45200.017.322817145
GATCGGA16350.015.9629926
TGCTCGG36800.015.9575695
ATCGGAG22900.015.5127997
TATACTG204800.015.398875
TGGCTCG38250.014.9735234
CTATACT125500.014.5574444
GTATAGG70100.014.1714891
CGGCTCG17150.013.9501284
GTGCTAG85900.013.928481
GTATAGA126950.013.7085491
Produced by FastQC (version 0.11.5)