FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_4329.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_4329.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13658372
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162890.11926018708525436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA68700.078.935986
TCGGAGA78700.071.945978
CGGAGAT82200.070.117359
CTCGGAG81000.066.2333767
GGCTCGG72450.065.043685
GGGCTCG63200.064.239354
CGTGGGC73150.039.253451
TGGGCTC141000.029.3079813
GTGGGCT105500.028.0375122
TATACTG145550.015.6403925
GATCGGA19500.015.2433156
TACACTG102800.015.2331885
CTACACT81900.015.1370894
GTACTAG46150.014.4548321
CCTACAC70200.014.2518793
CTATACT99800.014.1655764
GTGTAGG67750.013.9133271
TATACCG30750.013.6745495
CTTACAC85050.013.5535463
TCTATAC109750.013.4097813
Produced by FastQC (version 0.11.5)