FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_341.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_341.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19449838
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216180.11114745531556613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA77600.086.232026
TCGGAGA84400.083.235758
CGGAGAT87050.081.451429
CTCGGAG90400.074.2629557
GGGCTCG70950.070.607874
GGCTCGG84400.070.436865
CGTGGGC72100.047.267621
GTGGGCT157400.022.8458142
TGGGCTC236100.021.709613
GATCGGA16950.020.9582466
TATACTG228150.017.0322845
TGGCTCG43850.016.6985684
CTATACT144950.016.4552754
TACACTG171450.015.5187655
GTATAGG80900.014.7889231
CTACACT130200.014.7557864
ATCGGAG26500.014.4997227
CCTATAC80050.014.4000633
CGGCTCG21400.014.2286264
GTGTAGG96250.013.9370921
Produced by FastQC (version 0.11.5)