FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30908.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30908.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13239886
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146330.11052209966158318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA46700.079.793876
TCGGAGA55800.070.028998
CGGAGAT55750.069.57199
GGCTCGG51750.061.080065
CTCGGAG62000.060.45367
GGGCTCG49350.055.6769454
CGTGGGC55150.037.733061
GTGGGCT106050.020.0984822
TGGGCTC154400.019.4390353
TACACTG109850.016.9611915
TATACTG156100.014.9546525
GTGTTAG101550.014.7800561
TACACAG138300.014.3107965
GTATAGG57800.014.3008781
TCTATAC100750.014.031733
TGCTCGG32600.014.01026255
CTATACT105050.014.0095214
CCTACAC62700.013.9907023
TACACCG18700.013.9567485
GTGTAAG87150.013.8942371
Produced by FastQC (version 0.11.5)