FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30907.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30907.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22963086
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG242190.10546927359850501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA77750.071.986056
TCGGAGA85600.068.772488
CGGAGAT92000.064.145919
CTCGGAG95600.058.7729037
GGCTCGG89700.055.2834475
GGGCTCG80700.052.2853434
CGTGGGC98300.030.2435461
TATACTG271700.016.837255
GTGGGCT186650.016.7021682
TACACTG201350.016.5989535
TGGGCTC267050.016.0715833
CTACACT149700.015.3521074
CCTACAC114150.014.9887573
CTATACT169450.014.974664
GTGTAGG115800.014.5898131
GTATTAG167900.014.2948531
TACACAG262950.014.0613965
GTATAGG97450.013.9887321
TCTATAC167850.013.6056333
GTATAGA174200.013.5281251
Produced by FastQC (version 0.11.5)