FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30905.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30905.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15000994
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA64350.071.090066
CGGAGAT76350.065.424189
TCGGAGA76450.064.010968
CTCGGAG74650.062.4466637
GGGCTCG60600.060.0561874
GGCTCGG71150.057.5707175
CGTGGGC72400.038.157731
GTGGGCT115900.025.2088242
TGGGCTC156450.024.5600013
TATACTG169650.017.0508945
TACACTG113150.016.146345
CTATACT115150.015.9917654
GTATAGA116600.015.6710511
GTATTAG115050.014.6847121
CTACACT92650.014.3196474
TCTATAC113550.014.0463553
ATACTGT220600.013.5071716
GTGTAGG78250.013.158311
TACACAG141900.013.1304265
GTGTAAG94550.012.9604861
Produced by FastQC (version 0.11.5)