FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30890.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30890.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18242917
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA108900.064.7764056
CGGAGAT124000.060.0455259
TCGGAGA125400.058.6813858
CTCGGAG129700.054.4441037
GGCTCGG123350.049.7824335
GGGCTCG112650.046.7879334
TGGGCTC186350.029.1785533
CGTGGGC141250.027.5668241
GTGGGCT148050.027.1780822
TACACCG63700.016.275285
CTACACT99700.015.9977244
TACACTG121850.014.5771435
TATACTG156450.014.3654085
GTGTAGG97750.013.8715731
CCTACAC98200.013.8058123
CTATACT120750.013.3289674
CCTATAC76150.013.3287673
GTATTAG126550.013.2358171
GTATAGG77350.013.2178161
TCTACAC123400.012.807763
Produced by FastQC (version 0.11.5)