FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30852.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30852.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14970274
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA64000.077.595236
CGGAGAT78950.064.921989
TCGGAGA78950.064.279198
CTCGGAG79750.061.998277
GGCTCGG71200.061.3988575
GGGCTCG63450.058.386484
CGTGGGC77650.034.7377241
GTGGGCT119950.023.0277042
TGGGCTC169050.022.9007173
TACACTG117850.017.286215
TATACTG156400.015.3431415
TATACCG26550.015.2913735
GTGTAGG75250.014.9356831
GTATTAG108400.014.3816621
TACACCG33400.014.3258525
CTTACAC90450.014.1067543
CCTACAC72000.013.7946623
CTACACT91200.013.5137874
GTGTAAG96200.013.4166721
TACACAG148200.013.061295
Produced by FastQC (version 0.11.5)