FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30852.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30852.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14147503
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA65700.081.21566
TCGGAGA75100.074.042998
CGGAGAT77050.072.827749
GGCTCGG69050.068.8756265
GGGCTCG61500.066.7218864
CTCGGAG82500.064.5016257
CGTGGGC68050.044.325651
TGGGCTC152550.027.4690843
GTGGGCT114200.027.1711312
TATACTG137900.016.4027165
CTATACT98950.015.6792384
CTACACT80900.015.3240854
GATCGGA19750.015.0502526
TACACTG102000.014.9260395
TGGCTCG39750.014.0436694
CCTATAC57550.013.8572123
GTGTAAG83200.013.7696751
TAGACTG88250.013.6369995
GTCCTAC45000.013.5349511
CTAGTAC41300.013.5166543
Produced by FastQC (version 0.11.5)