FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30849.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30849.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13220560
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144730.10947342623913058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA59450.085.730746
GGGCTCG48350.079.321154
GGCTCGG57400.078.9404455
TCGGAGA68200.078.1335758
CGGAGAT70300.076.212099
CTCGGAG67550.076.202247
CGTGGGC52950.052.998291
GTGGGCT100400.029.3175092
TGGGCTC132800.029.0978283
TACACCG27950.017.8977645
TATACTG133250.016.213645
CTACACT79000.016.0598244
CTATACT93350.015.9986284
GATCGGA14300.015.7166066
TACACTG96700.014.6947125
GTATAGA98800.014.3852031
CCTACAC60400.014.1636943
TCTATAC91300.014.1345083
GTGTTAG92200.013.4487551
GTGTAGG61700.013.2803691
Produced by FastQC (version 0.11.5)