FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30849.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30849.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15321049
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169210.11044282933890494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA75800.070.7769246
CTCGGAG86000.061.4551437
GGGCTCG70650.058.7990344
TCGGAGA93700.058.4164438
CGGAGAT95500.058.150469
GGCTCGG82450.057.594345
CGTGGGC74650.038.8532521
TGGGCTC141750.029.255043
GTGGGCT110250.027.4869672
GTATAGA120750.015.1925471
TACACTG101900.014.7985215
TATACCG37100.014.6560365
TACACCG46500.014.6556165
CTATACT116650.014.356714
GTGTAGG76050.014.2064071
TATACTG151250.014.1881415
GTATAGG75600.013.9073181
CCTAGAC50550.013.7682213
CCTACAC73000.013.7051553
CTACACT86900.013.5986434
Produced by FastQC (version 0.11.5)