FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30786.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30786.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11403700
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA47750.073.181916
TCGGAGA51750.068.36598
CGGAGAT53700.067.638429
CTCGGAG54000.063.7719767
GGGCTCG43000.059.1791464
GGCTCGG50950.058.482745
CGTGGGC53200.033.255791
TGGGCTC132300.019.7824153
GTGGGCT95050.019.7552012
GTGTAGG57550.015.49704551
TATACTG122350.014.9915525
CTATACT83250.014.3691074
CTACACT71800.014.1362314
CTTACAC68600.013.8445593
TACACTG87150.013.8092725
GTATAGA84500.013.5578071
CTAGGAC44800.013.4317323
CTAACAC82050.013.2539293
ATACTGT152500.013.02599056
CTAGACT56400.012.8543754
Produced by FastQC (version 0.11.5)