FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30783.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30783.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20053536
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA113600.085.32646
TCGGAGA128450.079.243458
CGGAGAT132200.077.598869
CTCGGAG129800.075.626557
GGCTCGG120050.071.62275
GGGCTCG107350.069.8305744
CGTGGGC105900.050.7361561
GTGGGCT167950.033.2817232
TGGGCTC232150.032.6967853
TACACTG157650.015.911475
TATACTG218950.015.4301165
TACACCG49350.015.4251835
CTACACT121900.014.3925844
CTATACT142850.014.1088514
TGGCTCG64100.013.9115074
GTATAGG85250.013.8641
TCTATAC139650.013.5496053
CTAGACT95800.013.2434044
GTATTAG151900.013.2226141
GATCGGA27350.012.9888026
Produced by FastQC (version 0.11.5)