FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_MG_30783.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_MG_30783.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14996024
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG177070.11807796519930883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA85100.073.777176
CGGAGAT94400.069.043739
TCGGAGA94400.068.506138
CTCGGAG100550.062.1529547
GGCTCGG89350.061.017865
GGGCTCG77650.059.1009524
CGTGGGC84650.038.203941
TGGGCTC165450.028.920743
GTGGGCT119300.028.1978232
TATACTG138300.015.9886335
TACACTG111750.014.0781595
TACACCG42300.013.7114475
TGGCTCG51500.013.6551574
CTATACT102800.013.61124
GTCTAAG61750.013.3865361
GTGTAGG74100.013.3082511
TAGACTG82900.013.2929925
TATACAG128600.013.07918555
GTGTAAG84300.012.988221
GTATAGG63550.012.7791731
Produced by FastQC (version 0.11.5)