FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n02_Lib_prep_NC_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n02_Lib_prep_NC_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTGCGCGGATATGTGTATAAGCCACAGATGTACACCACTGACAATTA11.0752688172043012No Hit
GCGGCGGCCACAGGCGGAGAATAGCCGGCTGCTAGGCAAGGATAGCTGGG11.0752688172043012No Hit
GTTTGGGGCCTCTACTATTGGGACTGGGTGTAGATTCTGGTTAGGGTGAG11.0752688172043012No Hit
ACACATTGAAAAGCATTTTCCAATTTAGGAGTAATCAGTATTGTGCCTCA11.0752688172043012No Hit
CAGCGGTAGTGGCCGTGCCTTTCCAGCACTTCGATCTTGTAGCCGTCCGG11.0752688172043012No Hit
GTGTACGGTGGGGGAGCGCACTTGGGAACCGAGCCAGGGAAGGAGGCCTT11.0752688172043012No Hit
GTTATGATTTCTCCACATTGTTGCCAACACTTGTTATTATCTGACTTTTT11.0752688172043012No Hit
GTTGCAGAGTCGAATTGGACATAGATTCACATTCCTTGATTAGTGTGGTT11.0752688172043012No Hit
TGATTAATGAAAACATCTTTGGCAACTGCTTTCGCTTAAGTTAGTCTGAC11.0752688172043012No Hit
AATAAAGGTCTCTCTTATGACAAAAACGAATACTTAAGAACCAAATCTTA11.0752688172043012No Hit
ACAAAATACTATAAAAAAGAAATTATTTGAAAATATCTCAGCAAGATTAA11.0752688172043012No Hit
TCCGATCTCCTCCGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAA11.0752688172043012No Hit
CCCTCGTGATCACCCCCCCTTGGCCACCCAAACTCCTTCCATAACAGGCA11.0752688172043012No Hit
TGCTACTCTAATTGCTAGGAGCAGCAGGAGATGCCAAAATTATCACAAAA11.0752688172043012No Hit
GTGGATAGGAGAGTGTAAATAGAAAGAATAATAAAGAAAAACAAACCTTG11.0752688172043012No Hit
GCGACGGCGCCCGAGATGATACCCAGCACCGAGGGCTTCTTGGCGACAAG11.0752688172043012No Hit
GTTTTTAGTCTTATCTGGGACGATGATATGTGGCGAGTTTTTCCGGCTTG11.0752688172043012No Hit
CTTATACCCATCTGACGCTGCCTAGGAAGAGGATAGTGTAGATGTCGGTG11.0752688172043012No Hit
GGCGTATATAAATTTACATGAACCTTCTCATCTATATACTCAACTAGAAC11.0752688172043012No Hit
CAATAATGTAAATCAAGGGATTGGAGGAGGTAATGATACTATAAAGATAA11.0752688172043012No Hit
ATGGAATTGCTGGCAAAAAATAAGGAATCAAAACAATGAAGTTGAATAGG11.0752688172043012No Hit
CTTAAACACACCAAATAAGAAGTCACTAGTACCAATCATCGTACTAAACA11.0752688172043012No Hit
CAATTCGATACTGCATATTTGGGTAACGTGTCTATGTTCGTGAACTTAAA11.0752688172043012No Hit
ATCCAGCAGATGATAGCTTCGCACCATTGCCCGATCGGACAGATGCAAAT11.0752688172043012No Hit
GCGATGGTGGGCGAAGAGATCGATGTCCCAGCCCTGCGCGCGCAGGAAGG11.0752688172043012No Hit
CCCAGGGGGCTGGCGATGGGTGGGAAGTGGTGTGCGGTAAGGGCGGGAGC11.0752688172043012No Hit
CACTTCCTTAAGGATGTAATGGAGTCAATGAACAAACAATAAATGGGGAA11.0752688172043012No Hit
ATCCTAAATATTCAACATTAAAACACCGACTCAAATCAACAAACAATAAA11.0752688172043012No Hit
TATATACTGAAAGTAAAGAGGTGGAAAAAGAGATTCCACACAAATAGAAA11.0752688172043012No Hit
ATGTCCGCCGGATCGAGGAATTGGTCGGGTGCCCGGTGGCGCTGCTTTCC11.0752688172043012No Hit
AGTTACATGAGATGGCACGTACTGTGGTGGAGCGAACTATTCCTTGTTGA11.0752688172043012No Hit
CAAGCTGACCGAAAAATATAATTAGAATAAACTGTTACAAATGTAAGTAA11.0752688172043012No Hit
TAATAGTACAAAAATATAGTTAGATAGAAGGTATAAAACTAGAATTAAGG11.0752688172043012No Hit
GGTCTATGATATAAATGTACTTCATCTCCAACATTGACTCTATCGTCAAC11.0752688172043012No Hit
CACGTCTCCCAGGACAGATCGGAAGAGCACACGTCTCCCAGGACAGATCG11.0752688172043012Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTCAAGTGCGAGGGCTTCTTTTTTTCTCGCGAGGATCAGAGCAACACGT11.0752688172043012No Hit
GATATGAATTACAGAATCTGCGAATACTCTTACACTTGTATGGAAGAGTA11.0752688172043012No Hit
AGCATGGCCAAGTAGATAGTAAAGTTTACTTAAAATAGATTAGATATCTA11.0752688172043012No Hit
AGAGTGACCAGAGTGGTATTTCAACGATGACTCCACGAACACTGGCGTGC11.0752688172043012No Hit
ACAACAATAAAATAAAAACAATTATATTATAAATAATAACATTTAAAATC11.0752688172043012No Hit
GGGTCGGTAAGTCCGTTGGGGCTAGCAGTCTCGGGGCGGGAATGCCCGTT11.0752688172043012No Hit
TGTTTGGACAGTATGGATAGCAAATAGTAGCACTTCCACAACTAACTCAA11.0752688172043012No Hit
GCGCGAGGCACAGGCCCGTGCAGAGCACGCCGCCCACCAGGCGCAGGAAA11.0752688172043012No Hit
AATGTAAATGAATAACATTAGAAAGTGATTTAATTAATTTTTTCCAACAA11.0752688172043012No Hit
CTTCCGATCTGGTGGGGGCGCCTTGAGAGAACTCTGCTGAAGGAACTAGG11.0752688172043012No Hit
TTTTAACAAATAGTCCAATCTGGCCCCGCGCCGGGCGCGGGACGGCGGGG11.0752688172043012No Hit
GTTTCGAACCCTCCCAGGGCAGACCATCGCGCACCATAAACCTGGGAGAC11.0752688172043012No Hit
GCAGGAGCTGTTAGGAAAGAATCTTGTTGTTGGCTAGCACCATTTGTATT11.0752688172043012No Hit
CTTCTAACCGAGGTCGAAACGTACGTACTCTCTATCATCCCGTCAGGCCC11.0752688172043012No Hit
AAATAGAACTGAATAAATAAAACAGTATTAACTATATGTTTAAAAGGCAA11.0752688172043012No Hit
GGGGCAAGGGTTTCGAGAGGGCAGCTGGCAAGGTGTGAATGGGAGGCAAG11.0752688172043012No Hit
GGACAACAAACACCGAAACTGGAGCACCGCAACTCAGCCCGATTGATGGG11.0752688172043012No Hit
CGTGTTATCTGATATTTGTATAATAGAAATCTTTTTTAACTTAGAAGTTA11.0752688172043012No Hit
GTTCACAGCAGCCTGGCTTTCAGCATCGAGCTAGCGGCTCCTCGCCGAGC11.0752688172043012No Hit
GCTATATAATATACTATCTTCACTTACAAAAGAGTTATGAAGATCAAAAC11.0752688172043012No Hit
AGCTAAAAGAGGCTACTCAAGTGGGTCTTCCCAGAATAAAAAACTTTACC11.0752688172043012No Hit
GGCTTGTCCTACGCGTAGTAGTTAACAGCAAAGTACAGACACCTTATGTA11.0752688172043012No Hit
AGTAAAAGGTGATCATATCTGCAAACTCGTATAGTACTAAATTAAATAGA11.0752688172043012No Hit
CTCAGATTCTTGTTGCTTGTTGCTATGGCTTGGTTGTTTGAACTGCGAAG11.0752688172043012No Hit
CCACAGGATAAAATAAAATATAAACCCATCAAAATGCTGTATTAAATCTA11.0752688172043012No Hit
AGTACAGCAGTGTTCGGGGCTCTGGAAGTCTCCCGGGCTGCGCTTGGTGA11.0752688172043012No Hit
TACATACATACATACATACATACATAAAAGAAAGACAAAAAGAAAAGAAA11.0752688172043012No Hit
GAAAGAGCTAAGATAAAAGTTAACACAAAAAATAAAAGCACCAAACAAAA11.0752688172043012No Hit
GCTTAATTTGACCCAACACGGGAAACCTCACCCGGCCCGGCACAAATCGG11.0752688172043012No Hit
ATCTTCTAGGTGGGAGAATTTTCAACACTGGACTTATCTTTTATTTCTAA11.0752688172043012No Hit
AAATTTTTCTACCTTAATTGTTTTAATTATATATTTAATATAATTAAAGA11.0752688172043012No Hit
ATGAGACGATGTTCGGCCAGGCGAACGCGATTACGGCGGTCCAGGAGGCC11.0752688172043012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGG11.0752688172043012No Hit
GAAGTAGTGCGCCATATTACAATCCACAAAAGTAAGCGCCTTGAAAAACC11.0752688172043012No Hit
GTTATCTAACTATTGAGCTTCCTATACAGTATAACTGCTTTGTACATGTT11.0752688172043012No Hit
GGCCTTTATCGTCGGCGCGCTGTTCGGCCTGAGCGAGGCGAAACTGCCAG11.0752688172043012No Hit
TACTCAGTGTGACCGATAGTGGATAGTACCGTGAGGGAATGGTGAAAAGT11.0752688172043012No Hit
CAAAATTACAATACTATACAATCCAATCACAAAAAGTATACAAGACAAAA11.0752688172043012No Hit
GGATGAATGAGGGAGTACGAATGGGCCTAATTTATATAATTCTAAATGTT11.0752688172043012No Hit
ATACCATGCTTCATACAAAACGCAACTAGCATTCTTGTCAAATAGATAAA11.0752688172043012No Hit
TGTCTAGACTGTCCTGGCATTTACCATCAAAGTATTATAAGTAGCTTCTT11.0752688172043012No Hit
GACCCAATCAGACAATATCCATTCCAAAAAATAAAATTAAAAATAAAAAG11.0752688172043012No Hit
TTTGTGACTCCTCTAAATATATTTTTGTCAGTCTTGTTAGAGGTTTATCA11.0752688172043012No Hit
ATCTAAATATTAGCGCTGATTGGAGAAATGGCATAAAGGTGCAGCTCAAT11.0752688172043012No Hit
CCAGACCTACTAGGGAGAGTTGAACCTTGCATCAGAGAGCACATCCTGGG11.0752688172043012No Hit
CATTTAATGTGTAATTTGAATGCACTTAGTGTAATGTTGTTCCCTGGCCT11.0752688172043012No Hit
CAAATAAAAACAAATACTAAAAAAATTACTAAAACCAACAAACCATATAT11.0752688172043012No Hit
AGTATGCAGTTCGTTTTAATTCTCTAACACCAGAACAACAAAGAGATGTA11.0752688172043012No Hit
GATCTAGATAAGTATCGAAACGTGGCGGGCTGGATCTCATACTACTTAAA11.0752688172043012No Hit
GAAGAGGAAGCCTCAATTCAAACTCACTAGAAAAATATAAAGTGTGCTGT11.0752688172043012No Hit
GAAGAGTACACGTCTCCTTATTGAGATCGGAAGAGCACACGTCTTCCCCA11.0752688172043012Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGGCATTACTAAGTGGAAGGAAATCGAGGAGACCACCCGCCACGTCGCCC11.0752688172043012No Hit
CGTGTCGGGGTTTGTGTAAAATAACCGTGCATATGGTGTTTAGATTGCTC11.0752688172043012No Hit
AACAAAGACATTACATCACAGAAACATAACAAACCATCAGCTGTCACATC11.0752688172043012No Hit
CTTAAGACTTGGTTTCCCTTCGGCTCCGTGCCTTTCGCACTTAACCTCGC11.0752688172043012No Hit
CACGTCTCCCACATTAGATCGGAAGAGCACACGTCTCCCAGTTTAGATCG11.0752688172043012Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATATTTAGGTGGTGGTGCTGTAGTAGAATGCTTAGGTTAGTGTGTTGCTT11.0752688172043012No Hit
GTGCTGCGCTGGTCGTAGTCCTCTGTACAAGGGGGGCAATAGCCACGCAG11.0752688172043012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGTA50.0145.02
GTACACG50.0145.06
GAAGAGT50.0145.01
TACACGT50.0145.07
AGTACAC50.0145.05
AGAGTAC50.0145.03
GAGTACA50.0145.04
TGCAGTG50.0145.0145
ACACGTC150.048.3333328
CACGTCT150.048.3333329