FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C3_P2CW_und_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C3_P2CW_und_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926072
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG41540.2156721036389086No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA35120.18234001636491262No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT34260.17787497040609074No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA32900.17081396749446542No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG27950.1451139936617115No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT24050.12486552942984477No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT23850.12382714664872341No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA22530.11697382029332236No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT21560.11193766380488371No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA21480.11152231069243518No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA21360.11089928102376236No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT20730.10762837526323003No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA20570.10679766903833295No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT19360.10051545321254865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6150.0123.7911451
GCAAAAG39450.095.383493
GCAGGGT18950.078.428699
AGCAGGC3350.077.9090658
AGCGAAA12300.073.68521
GCAGGGG15450.071.7948469
AGCAGGG39750.065.476838
AGCAAAA60100.063.6960222
AAGCAGG72150.062.5006187
AGGGTGT7200.060.418736
CAAAAGC62800.060.26484
TTAGTAG1400.056.9662322
ACAAGGG9550.056.9391253
GAGCAAA67750.056.8277741
CAAGGGT9750.055.771144
AAGGGTG7850.055.415915
ATTAGTA1550.051.4560431
CGTGGAG450.00963819148.33624145
CAAGGTA4550.044.6145948
TCGTACT6000.043.5014882
Produced by FastQC (version 0.11.5)