FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C3_P2CW_und_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C3_P2CW_und_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803245
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG42000.23291344215566936No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA34840.1932072458262743No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA34670.1922645009413585No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT34360.1905453779159238No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG29040.16104300857620568No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT24200.13420250714683807No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT22120.12266774620198585No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA21040.11667854340369722No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT19950.11063388502394296No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA19920.11046751827954604No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA19680.10913658432437079No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT19230.10664108315841718No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA19170.10630834966962337No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT18900.10481104897005122No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT18800.10425649315539486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6550.0126.187361
GCAAAAG36400.097.593953
GCAGGGG13050.081.667089
GCAGGGT17150.076.093699
AGCGAAA11700.070.019782
AGCAGGG35900.066.239898
GCGCGAA451.1939231E-464.446561
CGCGAAA451.1942508E-464.442992
AGCAAAA55800.064.1831362
AAGCAGG68500.064.033187
AGCAGGC3300.063.712458
TACTTCG350.003565719662.14835145
ACGGCCG954.5656634E-1061.05803145
CAAAAGC59350.060.3440444
AGGGTGT7350.060.168716
TAAACTA4500.059.6097685
CTATTCG4700.058.6173259
GAGCAAA62600.057.677621
ACAAGGG9200.055.1617973
GCGGATA400.00604955654.379803145
Produced by FastQC (version 0.11.5)