FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C3_P2CW_100d_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C3_P2CW_100d_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006414
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG44120.21989479738478696No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA28270.14089813966609085No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA27650.13780804958498097No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT26350.13132882844716995No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG25890.1290361809676368No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT25240.12579657039873127No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT24970.12445088600857052No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA24680.12300552129321267No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA23340.11632693950500744No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC22640.1128381281231092No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA21780.10855187413963419No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA21650.10790395202585308No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT21520.10725602991207199No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG20760.10346817755458246No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA20450.10192313251402751No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT20150.10042792763607113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5300.0123.1245041
GCAAAAG34550.090.2330863
GGCGTAC259.3847135E-487.007981
GCGTACT259.3875016E-487.001472
AGCGAAA11400.075.6867751
GCAGGGT21350.074.7103969
TTACGTG300.001935322472.50666145
AGCAGGC4600.066.1984258
AGCAGGG35650.063.8596238
GCAGGGG10850.063.4819269
GTTGACG350.003565729862.14856145
TAAACTA5000.060.9010355
AGCAAAA52700.059.9820442
AGGGTGT10350.058.8445136
CTATTCG5200.058.5601469
AAGCAGG66350.057.585767
ACAAGGG13350.057.0234453
CAAGGGT13500.055.8528064
TTAGTAG1700.055.4462621
GCTCGTT400.006049573454.37999145
Produced by FastQC (version 0.11.5)