FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C2_P2CW_und_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C2_P2CW_und_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615282
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG32510.20126516608245495No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT28290.17513969696932175No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA26320.16294368413688756No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA21010.13007016731443796No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG19810.12264112396473187No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA18500.11453108497463602No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT18230.11285955022095213No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT18220.11279764152637124No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT17820.11032129374313587No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT17790.11013556765939322No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA17770.11001175027023145No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC17560.10871166768403287No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA17280.10697822423576812No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT16950.10493523731459893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4400.0108.761791
GCAAAAG25900.097.1317143
GCGCGAA406.1152423E-790.6348341
AGCGAAA9450.082.0988541
GCAGGGT14700.070.526129
GCAGGGG12550.068.166289
AGCAAAA38350.065.976822
CGCGAAA554.0631057E-665.908082
AGCAGGC3000.060.4157378
AAGCAGG51550.058.646047
AGCAGGG32600.058.488378
CAAAAGC46200.054.7664764
TCGTACT4550.050.9882282
GAGCAAA51700.049.2268071
ACAAGGG10000.048.5742577
CAAGGGT10500.045.570738
AACAGAC3900.044.6146977
ACAGACT4050.042.9623038
AAGGGTG8700.042.4993449
TATTAGT1401.4140824E-841.4330641
Produced by FastQC (version 0.11.5)