FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C2_P2CW_und_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C2_P2CW_und_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902622
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG51040.2682613782453898No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT42610.2239541012350325No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA40490.21281158317311583No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG34030.17885843851274716No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT28390.14921513574425188No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT25310.13302694912599558No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA24710.12987340627828334No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT23910.12566868248133364No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA23570.12388167486763005No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA21810.11463128251434074No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT21270.1117930939513997No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT21250.11168797585647595No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC20720.10890234634099678No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA20560.10806140158160686No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA20340.1069051025374457No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA20150.10590648063567014No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT19650.1032785282625766No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA19270.1012812844590255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6950.0128.32731
GCAAAAG40600.093.755723
AGCAGGC4050.078.764048
GCAGGGT21300.075.902419
GCAGGGG17300.074.594059
AGCGAAA13500.067.676141
AGCAAAA57150.066.985722
AGCAGGG44750.064.8033148
TTAGTAG1400.062.1515541
AAGCAGG76300.061.3816077
CAAAAGC64700.059.505184
TAAACTA3350.058.4364055
CTACTAA753.7614336E-758.0035446
GAGCAAA67450.057.1911051
AGGGTGT7950.055.6323286
ACAAGGG9650.052.5938873
CTATTCG3600.052.360189
TCGTACT5200.051.589692
CAAGGGT9900.051.265764
GGTCGTT1700.051.175567
Produced by FastQC (version 0.11.5)