FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C2_P2CW_100d_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C2_P2CW_100d_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210733
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG49140.2222792168932205No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA33830.1530261682437454No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT31480.14239620976391087No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA31210.1411748953853767No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC29010.13122344489361673No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT28910.13077110623490035No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG27800.12575014712314875No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT27750.12552397779379057No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA26660.12059348641378222No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA25900.11715571260753786No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA24940.11281326148386078No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT24060.1088326812871568No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG23290.10534967361504081No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA22290.10082628702787717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5250.0120.153231
GCAAAAG32900.093.658853
AGCGAAA12600.082.8642961
GCAGGGG12400.064.8974469
GCAGGGT22800.064.5487069
ACGGCCG350.00356617562.146812145
ACAAGGG17000.060.561313
AGCAAAA50950.060.1938322
ATTAGTA1102.5465852E-1159.32331
AGGGTGT14250.059.0199666
CAAGGGT17550.058.6633764
AGCAGGC4750.056.4721688
AGCAGGG41000.055.6997228
ACTAATA1700.055.439698
AAGGGTG15450.054.9051635
AAGCAGG71700.051.7698827
CAAAAGC61850.051.2268374
TACTAAT1850.050.944587
CTACTAA1900.049.6072966
TTAGTAG1850.047.0310821
Produced by FastQC (version 0.11.5)